Gattuso only took charge of the Valencia in June – but he has now been fired with the club sitting 14th in the Spanish top-flight.
The Italian penned a two-year deal upon his arrival but he has been unable to stop the terrible performance over recent years at the club.
Valencia confirmed the news on Monday night, with a statement saying: “Valencia CF announces that this Monday, January 30, the club and the first team coach, Gennaro Gattuso, have decided by mutual agreement to end the contractual relationship that united the Italian coach with the VCF.
“The Club wants to thank the coach for his commitment and work in these months in charge of the first team and wish him the best of luck in his future.
“The team will return to training this Tuesday, January 31, under the orders of ‘Voro’ Gonzalez.”
Gattuso won just five of his 18 LaLiga games in charge.